Our manuscript titled "Single-Molecule Imaging Reveals the Mechanism of Bidirectional Replication Initiation in Metazoa" was officially accepted for publication in Cell. The project started in the summer of 2020 (that first glorious COVID summer) when Scott started imaging how GINS is recruited to replication origins. He later did the same for Cdc45. In 2021 Riki joined the project and focused on imaging firing factors (TopBP1, RecQL4, and finally DONSON). It took a while to explore various hypotheses, sort out various technical difficulties, develop new assays, and develop new tools for analyzing the data. We submitted the paper in November 2023, and after a somewhat bumpy review process, the paper was accepted on 13th of May 2024 :)
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Tomoko, a 1st year CSB graduate student, just started her 2-month rotation in our lab. She will be working with Riki on understanding the mechanism of the CMG helicase activation. We're delighted to double the Japanese contingent of our lab :)
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Scott has joined our lab from the Biophysics department as our newest graduate student.
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Riki is joining us after having recently completed his PhD at Osaka University in Japan.
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Jordan Valgardson - a first year student in our home department of Chemical and Systems Biology is now officially rotating in the lab. He's leveling up his Single-Molecule Skills already and we look forward to getting some science done together in the next 2 months.
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Travis Lantz from Chemical and Systems Biology is starting a 2-month stint in our lab as a visiting student. He will be working with Linda to purify fluorescent proteins for some proof-of-principle experiments. In the meantime they are battling the cloning gremlins. Hang in there Travis!
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Luke Lynch from the Biochemistry PhD program will be spending the next 10 weeks studying how the replicative CMG helicase is regulated during replication stress - and we couldn't be more excited!
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Our microscope is up and running and we can see individual DNA molecules! Next stop - real-time imaging of single replicative helicases by KERHMIT!
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After almost a month of delivery mishaps, various delays and a heroic effort by Linda and Taylor, our ultra-efficient -80C freezer is here. Time to ship our samples in from the Walter Lab at Harvard Medical School!
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Our Nikon Eclipse Ti2 with PerfectFocus, motorized TIRF angle control, and dual EMCCD configuration is up and running! We're still running some tests and optimizing alignment, but we're only days away from doing real experiments :)
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Current Category: Milestones
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